Roma revamp with Gianluca Mancini and lose Davide Zappacosta in the warm-up, as Lazio rely on Ciro Immobile, Joaquin Correa and Manuel Lazzari.
The Derby della Capitale kicks off at the Stadio Olimpico at 17.00 UK time (16.00 GMT).
You can follow the build-up and action as it happens from all today’s seven Serie A games on the LIVEBLOG.
Build up to the showdown with our special Tactical Preview of this Rome Derby.
Form usually goes out the window in this fixture, but after the opening weekend, Lazio are certainly feeling more confident thanks to their 3-0 victory away to Sampdoria.
Roma, on the other hand, fumbled a lead three times for their 3-3 draw with Genoa on Coach Paulo Fonseca’s competitive debut.
Lucas Leiva has shaken off a knock to start with Sergej Milinkovic-Savic and Luis Alberto, while former SPAL winger Lazzari plays on with a protective cast after fracturing a metacarpal bone in his hand.
Immobile and Correa lead the attack once again, with Jordan Lukaku and Wallace the only absentees.
Roma are missing Diego Perotti and Leonardo Spinazzola, but there are several changes from the side that drew 3-3 with Genoa.
Alessandro Florenzi was set to move from right-back to the left side of the attacking trio supporting Edin Dzeko, alongside Cengiz Under and Nicolò Zaniolo.
Instead, Davide Zappacosta was going to get his first start after leaving Chelsea, with Gianluca Mancini replacing Juan Jesus at centre-back.
However, Zappacosta suffered a calf problem in the warm-up and has been replaced by Justin Kluivert, moving Florenzi to right-back again.
Chris Smalling arrived on loan from Manchester United this week and is in the stands.
Lazio: Strakosha; Luiz Felipe, Acerbi, Radu; Lazzari, Milinkovic-Savic, Leiva, Luis Alberto, Lulic; Correa, Immobile
Lazio bench: Guerrieri, Proto, Patric, Berisha, Bastos, Parolo, Caicedo, Jony, Cataldi, Adekanye, Marusic, Vavro
Roma: Pau Lopez; Florenzi, Mancini, Fazio, Kolarov; Cristante, Pellegrini; Under, Zaniolo, Kluivert; Dzeko
Roma bench: Fuzato, Mirante, Juan Jesus, Gonalons, Schick, Cetin, Santon, Veretout, Pastore, Diawara, Antonucci, Zappacosta